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proteome profiler mouse cytokine array  (R&D Systems)
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R&D Systems proteome profiler mouse cytokine array
Proteome Profiler Mouse Cytokine Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Proteome Profiler Mouse Xl Cytokine Array Analysis, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cytokine array panel a  (R&D Systems)
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Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Mouse Cytokine Array Panel A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ary006  (R&D Systems)
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Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Ary006, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteome profiler mouse xl cytokine array kit  (R&D Systems)
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R&D Systems proteome profiler mouse xl cytokine array kit
Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Proteome Profiler Mouse Xl Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteome profiler mouse xl cytokine array kit/product/R&D Systems
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proteome profiler mouse xl cytokine array kits  (R&D Systems)
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R&D Systems proteome profiler mouse xl cytokine array kits
Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Proteome Profiler Mouse Xl Cytokine Array Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteome profiler mouse xl cytokine array kits/product/R&D Systems
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R&D Systems proteome profiler mouse xl cytokine array kit purchased r d systems
Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Proteome Profiler Mouse Xl Cytokine Array Kit Purchased R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse xl cytokine array kit r d systems ary028 e z n a  (R&D Systems)
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R&D Systems mouse xl cytokine array kit r d systems ary028 e z n a
Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Mouse Xl Cytokine Array Kit R D Systems Ary028 E Z N A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse xl cytokine array kit  (R&D Systems)
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Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) <t>Proteome</t> Profiler Mouse <t>Cytokine</t> Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.
Mouse Xl Cytokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) Proteome Profiler Mouse Cytokine Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.

Journal: Scientific Reports

Article Title: Ablation of lipocalin-2 reduces neuroinflammation in a mouse model of Krabbe disease

doi: 10.1038/s41598-024-82927-1

Figure Lengend Snippet: Galc and Lcn2 double KO brain has a dramatic reduction in microgliosis along with a significant downregulation of multiple pro-inflammatory cytokines. ( A ) Immunohistochemistry on brain cryosections and quantification show a significant decrease of CD68 level (green) in Galc −/− ; Lcn2 −/− compared to Galc −/− . ( B ) Whole brain RT-qPCR analysis of inflammatory cytokines shows a significant downregulation of the pro-inflammatory TNF-α, MMP-3, and MCP-1 transcripts in Galc −/− ; Lcn2 −/− versus Galc −/− . N = 3 for Galc +/+ and Lcn2 −/− . N = 5 for Galc −/− ; Lcn2 −/− and Galc −/− all with 2 technical replicates. Data are presented as mean values ± SEM; * P < 0.05 , ** P < 0.01 , *** P < 0.001 , and ns = not significant. ( C ) Proteome Profiler Mouse Cytokine Array, which can detect 111 different mouse cytokines simultaneously, shows that multiple cytokines such as CD40, CXCL5, CD93, and CX3CL1 are significantly downregulated in the Galc −/− ; Lcn2 −/− compared to Galc −/− at P40.

Article Snippet: The Proteome Profiler Mouse XL Cytokine Array analysis (R&D Systems #ARY028) was performed on whole brain lysates as previously described .

Techniques: Immunohistochemistry, Quantitative RT-PCR